Indigenous tribes of Florida like the Seminole and Tequesta ground the root and soaked it overnight; afterwards, they rinsed it with running water for several hours to remove the rest of the water-soluble toxin cycasin. The resulting paste was then left to ferment before being dried into a powder. The resulting powder could then be used to make a bread-like substance. By the late 1880s, several mills in the Miami area started to produce Florida arrowroot until their demise after World War I. Seeds generally fall close to the parent plant, although about five percent of seeds are found more than four meters away. Some authors believe that birds and small mammals are responsible for that dispersal. While such behavior has not been observed, marks on seeds, and the location of seeds under shrubs where birds perch and small mammals shelter indicate that the seeds have been carried there. The size of the seeds probably restricts how far birds can carry seeds.

The whole plant, except for the sarcotesta, the pulpy covering of the seeds, is very toxic, containing a toxin called cycasin which can cause liver failure that can lead to death, but if proper precautions are taken it can be leached with water due to it being a water-soluble molecule. The seeds also contain a toxic glycoside which causes headaches, vomiting, stomach pains and diarrhoea if ingested, and Beta-methylamino-alanine, which can cause central nervous system failure.